Platelet-activating factor content in human spermatozoa and pregnancy outcome.
نویسندگان
چکیده
OBJECTIVE To determine whether platelet-activating factor (PAF) content in human spermatozoa from an isolated population is related to fertilization and pregnancy outcome. DESIGN Prospective analysis of PAF content in human spermatozoa after a Percoll gradient wash and its relation to fertilization and pregnancy outcome. SETTING University-based reproductive genetics laboratory. SUBJECT(S) Couples undergoing assisted reproduction. INTERVENTION(S) Lipids extracted from Percoll gradient spermatozoa were quantitated for PAF content by a specific radioimmunoassay. MAIN OUTCOME MEASURE(S) The relation between spermatozoa-derived PAF levels and motility, concentration, morphology, and fertilization and pregnancy rates were determined by using regression analysis and the Student t-test. RESULT(S) Radioimmunoassay and regression analysis showed a significant and positive relation between PAF content in human spermatozoa and concentration and motility indices and implantation rate. Patients who became pregnant had a significantly higher PAF content in the spermatozoa used (7.285 pmol/10(6) cells) than did patients who did not become pregnant (2.990 pmol/10(6) cells). CONCLUSION(S) The PAF content in human spermatozoa has a significant and positive relation with motility and concentration indices and implantation rate. Pregnancy rates but not fertilization rates may be predicted by measuring PAF levels in an isolated subpopulation of human spermatozoa.
منابع مشابه
The effect of platelet activating factor on the motility and acrosome reaction of ram spermatozoa
Platelet activating factor (PAF) is a novel signaling phospholipids that in addition to platelet activation has many biological properties. The acrosome reaction, as an essential step in mammalian fertilization, can occur in response to several agents such as PAF. Therefore, the present study aimed to assess the effect of PAF on the motility and acrosome reaction of ram spermatozoa. Semen was c...
متن کاملProstaglandins, Histamine and Platelet Activating Factor: Different Mediators in Dithranol-Induced Skin Damage
Dithranol is a potent agent in treating psoriasis but its adverse effects on intact skin have limited its usage. There are many proposed mediators for its adverse effects including prostaglandins, histamine, platelet activating factor and free radicals. In this study we examined the effect of different agents (diazepam, terfenadine, indomethacin and garlic extract) on dithranol-induced skin dam...
متن کاملExpression and Clinical Significance of Activating Transcription Factor 3 in Human Breast Cancer
Objective(s): Breast cancer is the most common type of cancer among women worldwide. This study investigated the expression and clinical significance of activating transcription factor 3 (ATF3) in human breast cancer and its relationship with the clinical outcome of breast cancer. Materials and Methods : ATF3 expressions were detected in 114 primary breast cancer tissues and ...
متن کاملP 143: The Effect of Platelet Activating Factor on Inflammatory Response in Multiple Sclerosis
Multiple sclerosis is an autoimmune disease of the central nervous system which its main characteristic is an inflammation and demyelination and subsequent, neural degeneration. Many studies have shown that inflammation causing neuronal demyelination. MS is the most common cause of chronic neurological disability in during youth which the prognosis is that can be death. Platelet activating fact...
متن کاملEffect of Follicular Fluid and Platelet-Activating Factor on Lactate Dehydrogenase C Expression in Human Asthenozoospermic Samples
Background: Application of follicular fluid (FF) and platelet-activating factor (PAF) in artificial insemination improves sperm motility. Lactate dehydrogenase C (LDH-C) is a key enzyme for sperm motility. In this study, the effects of FF and PAF on the sperm motility index and LDH-C expression were investigated. Moreover, LDH-C expression was compared between asthenozoospermic and normozoosper...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Fertility and sterility
دوره 74 2 شماره
صفحات -
تاریخ انتشار 2000